6533b856fe1ef96bd12b30ea

RESEARCH PRODUCT

Isolation of a high spin form of cytochrome P-450 induced in rat liver by 3-methylcholanthrene.

C.r. WolfFranz Oesch

subject

MaleImmunodiffusionCytochromeBiophysicsEnzyme-Linked Immunosorbent AssayBiochemistrychemistry.chemical_compoundCytochrome P-450 Enzyme SystemmedicineAnimalsInducerMolecular BiologybiologyRats Inbred StrainsCell BiologyOuchterlony double immunodiffusionRatsImmunodiffusionchemistryBiochemistryIsosafroleEnzyme InductionMethylcholanthreneMicrosomebiology.proteinMicrosomes LiverPhenobarbitalmedicine.drugMethylcholanthrene

description

Abstract A form of cytochrome P-450 (P-450 MC1) has been isolated from the livers of 3-methylcholanthrene-treated rats. The molecular weight is 54,500 and the heme iron is in the high spin configuration which clearly differenciates this form from the other major cytochrome induced by 3-methylcholanthrene (P-450 MC2). Whilst MC2 actively dealkylated 7-ethoxycoumarin and 7-ethoxyresorufin, MC1 was only active with 7-ethoxyresorufin. Ouchterlony immunodiffusion analysis and ELISA showed that anti MC1 and anti MC2 reacted with both MC1 and MC2 but preferentially with the homologous antigen. Both anti MC1 and MC2 cross-reacted strongly with microsomes from 3-methylcholanthrene, Aroclor 1254 and isosafrole-treated rats and also, but much weaker, with microsomes from phenobarbital, trans -stilbene oxide and chlofibrate-treated as well as untreated rats. Both MC1 and MC2 are induced by the same inducers, 3-methylcholanthrene, Aroclor 1254 and also isosafrole, whilst phenobarbital, trans -stilbene oxide and chlofibrate did not induce either of them, which shows that MC1 and MC2 are under similar control by various types of inducers, but MC1 was present in control microsomes at higher levels than MC2.

yearjournalcountryeditionlanguage
1983-03-01Biochemical and biophysical research communications
10.1016/0006-291x(83)90335-2https://pubmed.ncbi.nlm.nih.gov/6404267