6533b856fe1ef96bd12b32c0
RESEARCH PRODUCT
Photoaffinity labeling of the coupling factor 1 from the thermophilic bacterum PS3 by 8-azido ATP
Hans-jochen SchäferYasuo KagawaKlaus DoseGabriele RathgeberPeter Scheurichsubject
chemistry.chemical_classificationPhotoaffinity labelingStereochemistryNoncatalytic nucleotide binding siteThermophileBiophysicsCell BiologyBiochemistryCoupling (electronics)HydrolysisEnzymechemistryStructural BiologyPhotoaffinity labelingMoleBacterial F1ATPaseGeneticsUltraviolet lightCatalytic nucleotide binding siteNucleotideThermophilic bacterium PS3Molecular Biologydescription
AbstractTo localize the nucleotide binding sites of the F1ATPase (TF1) from the thermophilic bacterium PS3 we have used 14C-labeled 8-azido ATP (8-N3ATP) as photoaffmity label. 8-N3ATP is hydrolyzed by the F,ATPase in the absence of ultraviolet light. Irradiation by ultraviolet light of the enzyme in the presence of 8-N3ATP results in reduction of ATPase activity and in preferential nucleotide specific labeling of the α subunits (0.8–0.9 mol 8-N3ATP/TF1,α:β = 4:1). Inactivation and labeling do not depend on the presence of Mg2+. Both effects decrease upon addition of various nucleotide di- or triphosphates.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1984-08-20 | FEBS Letters |