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RESEARCH PRODUCT

Adventitious bud formation on isolated leaves and its significance for mutation breeding

subject

description

Since a mutation is a unicellular event, irradiation of a multicellular meristem results in the so-called diplontic selection. This competition between the mutated cell and the surrounding non-mutated cells is often lost by the mutated cell, causing a low frequency of mutated plants and a narrow mutation spectrum. When a mutated cell survives, chimeras are automatically formed because most apices consist of a number of fairly independent groups of cell layers. Such an undesirable situation can be improved by growing complete plants from only one cell, resulting in a high frequency of solid, non-chimeral mutants and a wide mutation spectrum. Many plant species can be stimulated to form adventitious buds on isolated leaves and in a number of cases it has been reported that only one cell was involved. From study of literature a list has been prepared in which the various plant species which can be stimulated to form adventitious buds on isolated leaves are grouped, according to the location type of the “new-formations” and giving the bibliographical references from which the data have been taken.