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RESEARCH PRODUCT

Chromosomal location polymorphism of major rDNA sites in two Mediterranean populations of the killifih Aphanius fasciatus (Pisces: Cyprinodontidae)

Angelo LibertiniRainer BarbieriAntonio MazzolaMariastella ColombaSalvatrice VizziniRoberto Vitturi

subject

Mediterranean climateMaleSilverAphanius fasciatuAphaniusGeneral Physics and AstronomyZoologyrDNABiologyDNA RibosomalChromosomesKaryotype polymorphismMediterranean seaStructural BiologyPolymorphism (computer science)FundulidaeNucleolus Organizer RegionAnimalsGeneral Materials Science18s rdnaKillifishIn Situ Hybridization FluorescenceGeneticsPolymorphism GeneticStaining and LabelingCell BiologyRibosomal RNAbiology.organism_classificationGenetics PopulationItalyKaryotypingMediterranean seaFemaleIn situ hybridizationMediterranean killifish

description

The chromosomes of the Mediterranean killifish, Aphanius fasciatus from two populations, the Lagoon of Venice (LV, 15 specimens) and the Lagoon ‘Stagnone di Marsala’ (Sicily) (SM, 48 specimens), have been investigated using conventional Ag-staining and fluorescent in situ hybridization (FISH) with 18S rDNA probe. The two methods revealed variation in the number of major rDNA sites ranging from 8 to 14 (LV) and from 1 to 4 (SM) per individual. The fact that each individual possessed its own number of sites implies that observed variation was structural. Moreover, overlapping of silver staining and FISH patterns demonstrated that all ribosomal genes were transcriptionally active in each specimen. q 2005 Elsevier Ltd. All rights reserved.

yearjournalcountryeditionlanguage
2005-04-01
10.1016/j.micron.2004.11.006http://hdl.handle.net/10447/20643