Arabidopsis thaliana nicotianamine synthase 4 is required for proper response to iron deficiency and to cadmium exposure.

6533b857fe1ef96bd12b3c24

RESEARCH PRODUCT

Arabidopsis thaliana nicotianamine synthase 4 is required for proper response to iron deficiency and to cadmium exposure.

Jeremy Astier Olivier Lamotte Frédéric Gaymard Emmanuel Koen Jossia Boucherez Angélique Besson-bard David Wendehenne Pierre Richaud Céline Duc Antoine Gravot

subject

0106 biological sciences [ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology MESH : Azetidinecarboxylic Acid FMN Reductase Arabidopsis thaliana Mutant Arabidopsis Gene Expression Plant Science 01 natural sciences MESH : Cation Transport Proteins MESH : Iron MESH : Arabidopsis Proteins Nicotianamine synthase MESH : Plants Genetically Modified chemistry.chemical_compound MESH : Arabidopsis Gene Expression Regulation Plant Gene expression MESH: Genes Plant Arabidopsis thaliana MESH : DNA Bacterial Homeostasis MESH: Arabidopsis Nicotianamine MESH: Stress Physiological Cation Transport Proteins MESH : Adaptation Physiological MESH : Cadmium 2. Zero hunger chemistry.chemical_classification 0303 health sciences Cadmium MESH: Iron biology General Medicine Iron Deficiencies Plants Genetically Modified Adaptation Physiological MESH: Azetidinecarboxylic Acid MESH : Phenotype Phenotype Biochemistry MESH: Homeostasis MESH : Homeostasis MESH : Mutation Azetidinecarboxylic Acid Cadmium DNA Bacterial MESH: Gene Expression MESH: Mutation Iron MESH: Cadmium chemistry.chemical_element MESH: Ferritins MESH: Arabidopsis Proteins MESH: Alkyl and Aryl Transferases Genes Plant MESH: Phenotype Nicotianamine synthase 03 medical and health sciences MESH: Cation Transport Proteins Stress Physiological Iron homeostasis Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Iron deficiency (plant disorder)MESH: Gene Expression Regulation Plant MESH : Genes Plant MESH : Alkyl and Aryl Transferases MESH : Stress Physiological 030304 developmental biology MESH : FMN Reductase Alkyl and Aryl Transferases Arabidopsis Proteins Iron deficiency Nitric oxide Nicotianamine biology.organism_classification MESH: Adaptation Physiological MESH: DNA Bacterial MESH : Gene Expression Enzyme chemistry MESH: FMN Reductase MESH: Plants Genetically Modified Ferritins Mutation biology.protein MESH : Ferritins Agronomy and Crop Science MESH : Gene Expression Regulation Plant 010606 plant biology & botany

description

International audience; The nicotianamine synthase (NAS) enzymes catalyze the formation of nicotianamine (NA), a non-proteinogenic amino acid involved in iron homeostasis. We undertook the functional characterization of AtNAS4, the fourth member of the Arabidopsis thaliana NAS gene family. A mutant carrying a T-DNA insertion in AtNAS4 (atnas4), as well as lines overexpressing AtNAS4 both in the atnas4 and the wild-type genetic backgrounds, were used to decipher the role of AtNAS4 in NA synthesis, iron homeostasis and the plant response to iron deficiency or cadmium supply. We showed that AtNAS4 is an important source for NA. Whereas atnas4 had normal growth in iron-sufficient medium, it displayed a reduced accumulation of ferritins and exhibited a hypersensitivity to iron deficiency. This phenotype was rescued in the complemented lines. Under iron deficiency, atnas4 displayed a lower expression of the iron uptake-related genes IRT1 and FRO2 as well as a reduced ferric reductase activity. Atnas4 plants also showed an enhanced sensitivity to cadmium while the transgenic plants overexpressing AtNAS4 were more tolerant. Collectively, our data, together with recent studies, support the hypothesis that AtNAS4 displays an important role in iron distribution and is required for proper response to iron deficiency and to cadmium supply.

year	journal	country	edition	language
2013-08-01

10.1016/j.plantsci.2013.04.006 https://hal.archives-ouvertes.fr/hal-00967003