6533b857fe1ef96bd12b3d2d

RESEARCH PRODUCT

25S rDNA-based molecular monitoring of glomalean fungi in sewage sludge-treated field plots

D. Van TuinenOdile ChatagnierSilvio GianinazziVivienne Gianinazzi-pearsonE. Jacquot-plumey

subject

0106 biological sciencesSewageRoot systemPlant RootsPolymerase Chain Reaction01 natural sciencesMicrobiologyGLOMALESADN RIBOSOMIALSoil PollutantsDNA FungalSymbiosisRelative species abundance[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologySoil MicrobiologyComputingMilieux_MISCELLANEOUSEcology Evolution Behavior and Systematics2. Zero hungerPollutantSewagebiologybusiness.industryEcologyfungiFungiAgricultureFabaceae04 agricultural and veterinary sciences15. Life on landContaminationbiology.organism_classificationArbuscular mycorrhizaBiodegradation Environmental[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyRNA Ribosomal13. Climate actionEnvironmental chemistrySoil water040103 agronomy & agriculture0401 agriculture forestry and fisheriesbusinessSludgeEnvironmental Monitoring010606 plant biology & botany

description

Recycling of sewage wastes in agriculture is likely to affect the biological activity of soils through contamination of ecosystems by pathogens and metallic or organic micropollutants. The impact of sewage sludge spreading under field conditions on arbuscular mycorrhiza (AM) formation by a community of glomalean fungi was evaluated using a nested polymerase chain reaction (PCR) and discriminating primers based on 25S rDNA polymorphisms to detect different fungal species within root systems. Medicago truncatula was grown in soil of field plots amended or not with a composted sewage sludge, spiked or not with organic or metallic micropollutants. Overall AM development in roots decreased with sewage sludge application, and the relative abundance of five AM fungal morphotypes in root fragments was modified by the input of composted sludges. Sewage sludge spiked or not with organic pollutants had a generally positive effect on the relative diversity of AM fungal populations in planta, whereas after spreading of the sludge spiked with metallic pollutants, no variation was observed in the abundance of different species.

yearjournalcountryeditionlanguage
2001-01-01
https://hal.inrae.fr/hal-02674387