A Genomic, Transcriptomic and Proteomic Look at the GE2270 Producer Planobispora rosea, an Uncommon Actinomycete.

6533b857fe1ef96bd12b45e3

RESEARCH PRODUCT

A Genomic, Transcriptomic and Proteomic Look at the GE2270 Producer Planobispora rosea, an Uncommon Actinomycete.

Silke Alt Luca Petiti Gianluca De Bellis Andrea Scaloni Roberta Bordoni Clelia Peano Anna Maria Puglia Arianna Tocchetti João C. S. Cruz Giorgio Corti Anna Maria Salzano Margherita Sosio Giuseppe Gallo Stefano Donadio

subject

Proteome Sequence analysis lcsh:Medicine Genomics Biology Genome Peptides Cyclic 03 medical and health sciences Bacterial Proteins Planobispora rosea Actinomycetales Genomic library Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)lcsh:Science Gene Proteomic Look 030304 developmental biology Whole genome sequencing Genetics 0303 health sciences Multidisciplinary Biochemistry Genetics and Molecular Biology (all)030306 microbiology Shotgun sequencing Sequence Analysis RNA Medicine (all)lcsh:R Uncommon Actinomycete.High-Throughput Nucleotide Sequencing Genomics RNA Bacterial Thiazoles Glucose Transcriptomic Agricultural and Biological Sciences (all)Multigene Family Proteome Genomic lcsh:Q Transcriptome Genome Bacterial Metabolic Networks and Pathways Research Article

description

We report the genome sequence of Planobispora rosea ATCC 53733, a mycelium-forming soil-dweller belonging to one of the lesser studied genera of Actinobacteria and producing the thiopeptide GE2270. The P. rosea genome presents considerable convergence in gene organization and function with other members in the family Streptosporangiaceae, with a significant number (44%) of shared orthologs. Patterns of gene expression in P. rosea cultures during exponential and stationary phase have been analyzed using whole transcriptome shotgun sequencing and by proteome analysis. Among the differentially abundant proteins, those involved in protein metabolism are particularly represented, including the GE2270-insensitive EF-Tu. Two proteins from the pbt cluster, directing GE2270 biosynthesis, slightly increase their abundance values over time. While GE2270 production starts during the exponential phase, most pbt genes, as analyzed by qRT-PCR, are down-regulated. The exception is represented by pbtA, encoding the precursor peptide of the ribosomally synthesized GE2270, whose expression reached the highest level at the entry into stationary phase. Copyright:

year	journal	country	edition	language
2015-01-01	PLoS ONE

10.1371/journal.pone.0133705 http://europepmc.org/articles/PMC4514598?pdf=render