6533b857fe1ef96bd12b4710

RESEARCH PRODUCT

6-Hydroxydopamine lesioning differentially affects α-synuclein mRNA expression in the nucleus accumbens, striatum and substantia nigra of adult rats

Peter JennerBai-yun ZengA. OwenCarla CannizzaroBanu C. TelB. DassStephen E. Rose

subject

Malemedicine.medical_specialtyTyrosine 3-MonooxygenaseDopamineanimal diseasesDopamine AgentsSynucleinsNerve Tissue ProteinsSubstantia nigraStriatumNucleus accumbensBiologyDrug Administration ScheduleNucleus Accumbenschemistry.chemical_compoundDopamineInternal medicineBasal gangliamedicineAnimalsTyrosine hydroxylase mRNARNA MessengerRats WistarOxidopamineNeuronsHydroxydopamineTyrosine hydroxylaseGeneral NeuroscienceParkinson Diseaseα-Synuclein mRNARatsnervous system diseasesNeostriatumSubstantia NigraEndocrinologynervous systemchemistrySympatholyticsalpha-SynucleinSettore BIO/14 - Farmacologia6-HydroxydopamineOxidopaminemedicine.drug

description

The effect of a unilateral 6-hydroxydopamine (6-OHDA) lesion and/or repeated administration of levodopa (L-DOPA) to normal and 6-OHDA-lesioned rats on alpha-synuclein mRNA expression was investigated by in situ hybridization histochemistry. A 6-OHDA lesion of the nigro-striatal pathway alone, confirmed by the loss of nigral tyrosine hydroxylase mRNA expression, markedly decreased alpha-synuclein mRNA in the lesioned substantia nigra (SN). In contrast, the levels of alpha-synuclein mRNA in the denervated striatum and nucleus accumbens were not altered. Chronic administration of L-DOPA to normal or 6-OHDA-lesioned rats had no effect on alpha-synuclein mRNA expression in the SN, striatum or nucleus accumbens. These data confirm that alpha-synuclein is localized in the nigro-striatal tract but that its gene expression is not regulated by dopamine.

yearjournalcountryeditionlanguage
2002-04-18Neuroscience Letters
https://doi.org/10.1016/s0304-3940(02)00083-6