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RESEARCH PRODUCT

Karyotype Abnormalities in a Variant Chinese Hamster Cell Line Resistant to Methyl Methanesulphonate

Fabio CaradonnaGiusi BarbataGiulia Sciandrello

subject

Alkylating AgentsCellPopulationDrug ResistanceChromosome DisordersChromosomal translocationChinese hamsterCell LineCricetulusCricetinaeGene duplicationGeneticsmedicineAnimalseducationChromosome AberrationsGeneticseducation.field_of_studybiologyChromosome MappingChromosomeKaryotypeGeneral MedicineMethyl Methanesulfonatebiology.organism_classificationMolecular biologyChromosome Bandingmedicine.anatomical_structureCell cultureKaryotyping

description

A variant cell population, isolated from V79-CI 3 Chinese hamster cells after two consecutive treatments with methyl methanesulphonate (MMS), was found to be highly resistant to killing by this alkylating agent. The resistant cell line was cytogenetically characterized both by the presence of a stable translocation involving metacentric chromosome 2 and acrocentric chromosome 6 and by a supernumerary chromosome originated by the duplication of a small telocentric chromosome. This cell population also showed a transient transformed phenotype, seen as formation of transformed foci containing cells with high chromosomes counts and multiple chromosomal aberrations. As MMS-resistance and karyotype changes are permanent and heritable traits, we suppose that they are related events.

yearjournalcountryeditionlanguage
2004-05-11Hereditas
https://doi.org/10.1111/j.1601-5223.1996.00039.x