6533b857fe1ef96bd12b5045
RESEARCH PRODUCT
Siderophores and related outer membrane proteins produced by pseudomonads isolated from eels and freshwater.
Elena AlcaideRosa Aznarsubject
SiderophoreIronSiderophoresFresh WaterMicrobiologyMicrobiologychemistry.chemical_compoundEnterobactinSpecies SpecificityArthrobacterPseudomonasGeneticsAnimalsMolecular BiologyEelsbiologyPseudomonasbiology.organism_classificationMolecular WeightchemistryMembrane proteinBiochemistryBacterial outer membraneWater MicrobiologyBacteriaPseudomonadaceaeBacterial Outer Membrane Proteinsdescription
A total of 46 environmental pseudomonads, together with six type strains, were examined for their siderophore-producing activity. All strains were able to grow under iron-limiting conditions, gave orange halos in the CAS agar assay, and produced hydroxamates, and some of them also produced phenolate-type compounds. Bioassays showed that all strains, except Pseudomonas aeruginosa, promoted growth of mutant strain Arthrobacter flavescens JG-9, deficient in hydroxamate production, and some of them promoted growth of Salmonella typhimurium enb-1, which requires enterobactin for growth. The presence of iron-regulated outer membrane proteins was observed, the molecular size of the main induced proteins ranged between 76 and 93 kDa.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-11-01 | FEMS microbiology letters |