6533b858fe1ef96bd12b5931
RESEARCH PRODUCT
Methanotrophs are core members of the diazotroph community in decaying Norway spruce logs
Aino SmolanderRaisa MäkipääSonia Sanz MuñozTero TuomivirtaMarja TiirolaHannu FritzeSanna M. Leppänensubject
0106 biological sciences0301 basic medicineta1172Soil Sciencechemistry.chemical_element010603 evolutionary biology01 natural sciencesMicrobiologyMethane03 medical and health scienceschemistry.chemical_compoundlahoaminenBotanyDry matterlahopuutritsobitdead woodnifHbiologyPicea abiesChemistryta1183coarse woody debrisPicea abiesbiology.organism_classificationNitrogenSubstrate (marine biology)kuusi030104 developmental biologytypensidontaasymbiotic nitrogen fixationNitrogen fixationDiazotrophCoarse woody debrisdescription
Dead wood is initially a nitrogen (N) poor substrate, where the N content increases with decay, partly due to biological N2 fixation, but the drivers of the N accumulation are poorly known. We quantified the rate of N2 fixation in decaying Norway spruce logs of different decay stages and studied the potential regulators of the N2-fixation activity. The average rate for acetylene reduction in the decaying wood was 7.5 nmol ethylene g−1d−1, which corresponds to 52.9 μg N kg−1d−1. The number of nifH copies (g−1 dry matter) was higher at the later decay stages, but no correlation between the copy number and the in vitro N2 fixation rate was found. All recovered nifH sequences were assigned to the order Rhizobiales, and therein mostly (60%) to methane oxidizing genera. We confirm that nitrogen fixing methanotrophs are present in all the wood decay phases and suggest that their interaction between methane producing organisms in decaying wood should be further studied. peerReviewed
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-05-01 | Soil Biology and Biochemistry |