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RESEARCH PRODUCT
PCR for the detection of pathogens in neonatal early onset sepsis.
Paul T. HeathPhilip D. ButcherClarissa OeserMarcus PondPhilipp HennekeTim PlancheKen LaingAlison Bedford RussellKathryn A. Harrissubject
PhysiologyArtificial Gene Amplification and ExtensionPathology and Laboratory Medicinemedicine.disease_causePolymerase Chain ReactionUreaplasmaUreaplasmaMycoplasma0302 clinical medicineAntibioticsRNA Ribosomal 16SMedicine and Health Sciences030212 general & internal medicineAge of OnsetCandidaMultidisciplinaryNeonatal sepsisAntimicrobialsQCandidiasisRDrugsPneumococcusBacterial InfectionsBacterial PathogensBody FluidsBloodMedical MicrobiologyInfant Extremely PrematureMedicinePathogensNeonatal SepsisAnatomyInfant PrematureResearch ArticleStaphylococcus aureusScienceMycoplasma hominisBiologyResearch and Analysis MethodsReal-Time Polymerase Chain ReactionMicrobiologyDNA RibosomalSensitivity and SpecificityMicrobiology03 medical and health sciencesSigns and SymptomsEnterobacteriaceaeDiagnostic MedicineSepsisMicrobial Control030225 pediatricsStreptococcus pneumoniaemedicineHumansMolecular Biology TechniquesMicrobial PathogensMolecular BiologyPharmacologyBacteriaOrganismsInfant NewbornBiology and Life SciencesNeonatesStreptococcusMycoplasmamedicine.diseasebiology.organism_classificationEarly DiagnosisStreptococcus agalactiaeMultiplex Polymerase Chain ReactionEnterococcusDevelopmental BiologyUreaplasma urealyticumEnterococcus faeciumdescription
Background A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not detected by conventional culture. Methods Whole blood samples of 208 neonates with suspected early onset sepsis were tested using a panel of multiplexed bacterial PCRs targeting Streptococcus pneumoniae, Streptococcus agalactiae (GBS), Staphylococcus aureus, Streptococcus pyogenes (GAS), Enterobacteriaceae, Enterococcus faecalis, Enterococcus faecium, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium, a 16S rRNA gene broad-range PCR and a multiplexed PCR for Candida spp. Results Two-hundred and eight samples were processed. In five of those samples, organisms were detected by conventional culture; all of those were also identified by PCR. PCR detected bacteria in 91 (45%) of the 203 samples that did not show bacterial growth in culture. S. aureus, Enterobacteriaceae and S. pneumoniae were the most frequently detected pathogens. A higher bacterial load detected by PCR was correlated positively with the number of clinical signs at presentation. Conclusion Real-time PCR has the potential to be a valuable additional tool for the diagnosis of neonatal sepsis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-01-24 |