6533b858fe1ef96bd12b5a88

RESEARCH PRODUCT

Mandarin essential oils inhibit quorum sensing and virulence factors of Pseudomonas aeruginosa

Alicia BardónAlicia BardónElena CartagenaMaría Amparo BlázquezMario Eduardo ArenaMario Eduardo ArenaMaría Constanza Luciardi

subject

0301 basic medicinePseudomonas aeruginosaOtras Ciencias BiológicasBiofilm030106 microbiologyBiofilmVirulencePseudomonas AeruginosaBiologyAntipathogenicmedicine.disease_causeMicrobiologyCiencias Biológicas//purl.org/becyt/ford/1 [https]03 medical and health sciencesQuorum sensing030104 developmental biologyCitrus Reticulata Essential OilBotanymedicine//purl.org/becyt/ford/1.6 [https]CIENCIAS NATURALES Y EXACTASFood Science

description

Pseudomonas aeruginosa produces biofilm and several virulence factors coordinated by quorum sensing (QS) in food. The interruption of QS is a target to control the bacterial virulence. Chemical preservatives used to control biofilm give rise to several food safety problems. For this reason, essential oils (EOs), generally recognized as safe products, are a hopeful alternative. The aim of this work was to determine the chemical composition of mandarin EOs obtained by cold-pressing (EOP) and cold-pressing followed by steam distillation (EOPD) and their antipathogenic properties against P. aeruginosa. Both EOs contained the highest quantities of monoterpene hydrocarbons, mainly limonene, followed by γ-terpinene, myrcene and α-pinene. Although the EOs were not able to inhibit the bacterial growth at 4 mg/mL, EOP and EOPD significantly inhibited the P. aeruginosa biofilm formation at 0.1 mg/mL. In addition to the EOs inhibited biofilm cell viability (41%), AHL production (33%) and resulted in higher than 75% reduction in elastase enzyme activity. In conclusion, Citrus reticulata EOs are suitable alternatives to chemical additives for general use in the food industry. Fil: Luciardi, María Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina Fil: Blazquez, María Amparo. Universidad de Valencia; España Fil: Cartagena, Elena. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina Fil: Arena, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina

yearjournalcountryeditionlanguage
2016-05-01
10.1016/j.lwt.2015.12.056https://www.sciencedirect.com/science/article/pii/S0023643815304138