Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency

6533b858fe1ef96bd12b617e

RESEARCH PRODUCT

Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency

Mindaugas Tamošiūnas A. ĻIhačovs Audrius Kilikevicius Saulius ŠAtkauskas Roberts Kadikis Dainis Jakovels Juozas Baltusnikas

subject

Tibialis Cranialis animal structures viruses Genetic enhancement Electroporation fungi Transfection Biology Molecular biology Fluorescence spectroscopy Cell biology Green fluorescent protein chemistry.chemical_compound chemistry embryonic structures Sonoporation DNA

description

Electroporation and ultrasound induced sonoporation has been showed to induce plasmid DNA transfection to the mice tibialis cranialis muscle. It offers new prospects for gene therapy and cancer treatment. However, numerous experimental data are still needed to deliver the plausible explanation of the mechanisms governing DNA electro- or sono-transfection, as well as to provide the updates on transfection protocols for transfection efficiency increase. In this study we aimed to apply non-invasive optical diagnostic methods for the real time evaluation of GFP transfection levels at the reduced costs for experimental apparatus and animal consumption. Our experimental set-up allowed monitoring of GFP levels in live mice tibialis cranialis muscle and provided the parameters for DNA transfection efficiency determination.

year	journal	country	edition	language
2014-10-22	SPIE Proceedings

https://doi.org/10.1117/12.2083895

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