6533b858fe1ef96bd12b6319

RESEARCH PRODUCT

Fabrication of silver nanoparticles by a diethylene triamine-hyaluronic acid derivative and use as antibacterial coating

Annalisa MartoranaGiovanna PitarresiFabio Salvatore PalumboValentina CataniaDomenico SchillaciNicolò MauroCalogero FioricaGaetano Giammona

subject

DiethylenetriamineSilverPolymers and PlasticsAg nanoparticlesHyaluronic acidOrganic ChemistryDEETMetal NanoparticlesAnti-Bacterial AgentsSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoMaterials ChemistryPolyaminesAntibacterial activityMedical device infections

description

In this work a synthetic protocol for the functionalization of hyaluronic acid with diethylenetriamine (DETA) was standardized. HA-DETA derivatives were characterized by NMR and proton carbon correlation analysis HSQC and HMBC to confirm chemical structure. A selected derivative was used to set up a green fabrication procedure for HA-DETA capped silver nanoparticles with the aim to achieve a polymeric based coating with potential application in the treatment of medical devices associated infections. Data from UV-visible spectroscopy, electron scanning and transmission microscope (STEM), photoelectric spectroscopy (XPS) and rheological characterization were combined to characterize the HA-DETA/Ag nanocomposites. HA-DETA stabilized Ag nanoparticles (10-30 nm) were obtained through an UV accelerated production. The viability of MC3T3-E1 was analyzed with the aim of designing a cytocompatible antimicrobial coating. Antibacterial and antibiofilm activity of HA-DETA/Ag nanocomposites have been tested in vitro against Staphylococcus aureus and Pseudomonas aeruginosa both in culture plates than on titanium specimens.

yearjournalcountryeditionlanguage
2022-05-23
10.1016/j.carbpol.2022.119861http://hdl.handle.net/10447/565589