6533b858fe1ef96bd12b6333

RESEARCH PRODUCT

Direct conjugal transfers of Ti plasmid to soil microflora

Xavier NesmeS. Teyssier-cuvelleChristophe Mougel

subject

DNA BacterialAgrobacteriumSequence analysisAuxotrophy[SDV]Life Sciences [q-bio]Molecular Sequence DataMicrobial Sensitivity TestsPolymerase Chain ReactionMicrobiology03 medical and health sciencesTi plasmidRNA Ribosomal 16SGenetics[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyEcology Evolution Behavior and SystematicsPhylogenySoil MicrobiologyComputingMilieux_MISCELLANEOUS030304 developmental biologyDNA Primers0303 health sciencesbiologyBase Sequence030306 microbiologyDrug Resistance MicrobialSequence Analysis DNARibosomal RNAbiology.organism_classificationSinorhizobiumConjugation GeneticMicrobial geneticsSoil microbiologyPolymorphism Restriction Fragment LengthPlasmidsRhizobium

description

The bacterial species in soil that can receive a Ti plasmid by conjugation from Agrobacterium spp. were investigated. In order to have direct access to the potential reservoir of Ti plasmid amongst soil microflora, the conjugal system consisting of a multiply auxotrophic derivative of C58 (ST-96-4) and a derivative of pTiC58Delta(acc)R (pSTiEGK) containing a triple antibiotic-resistance cassette in traM was used to transfer the Ti plasmid in a complex soil microflora used as the recipient. Numerous transconjugants were obtained by this method but none was identified as Agrobacterium. This could be explained by the low density of Agrobacterium in the tested soil. As indicated by analysis of the ribosomal gene rrs, transconjugants recovered directly from soil were found to be new bacterial species which appeared to be closely related to Sinorhizobium spp.

yearjournalcountryeditionlanguage
2002-01-01
https://hal.inrae.fr/hal-02692194