6533b858fe1ef96bd12b63fb

RESEARCH PRODUCT

Structure of a polysaccharide from the lipopolysaccharide of Vibrio vulnificus clinical isolate YJ016 containing 2-acetimidoylamino-2-deoxy-L-galacturonic acid.

Sof'ya N. SenchenkovaSusana MerinoAlexander S. ShashkovConsuelo EsteveYuriy A. KnirelJuan M. TomásElena Alcaide

subject

chemistry.chemical_classificationLipopolysaccharidesMagnetic Resonance SpectroscopybiologyLipopolysaccharideChemistryStereochemistryOrganic ChemistryMolecular Sequence DataPolysaccharides BacterialGeneral MedicineVibrio vulnificusbiology.organism_classificationPolysaccharideBiochemistryAnalytical ChemistrySugar derivativesResidue (chemistry)chemistry.chemical_compound13c nmr spectroscopyBiochemistryCarbohydrate SequenceGalacturonic acidVibrio vulnificus

description

Abstract A polysaccharide isolated after mild acid degradation of the lipopolysaccharide of Vibrio vulnificus clinical isolate YJ016 was found to contain l -Fuc, d -GlcpNAc, 2,4-diacetamido-2,4,6-trideoxy- d -glucose (di-N-acetylbacillosamine, d -QuiNAc4NAc), and 2-acetimidoylamino-2-deoxy- l -galacturonic acid ( l -GalNAmA). The last sugar derivative was confirmed by correlations for nitrogen-linked protons in 2D TOCSY and ROESY spectra measured in a H2O–D2O mixture. The following structure of the polysaccharide was established by 1H and 13C NMR spectroscopy, including 2D ROESY and 1H,13C HMBC experiments: Download : Download full-size image where the degree of 6-O-acetylation of the lateral β-GlcNAc residue is ∼70%.

yearjournalcountryeditionlanguage
2009-01-19Carbohydrate research
10.1016/j.carres.2009.03.021https://pubmed.ncbi.nlm.nih.gov/19368903