6533b858fe1ef96bd12b640b
RESEARCH PRODUCT
Nuclear and Cytoplasmic Soluble Proteins Extraction from a Small Quantity of Drosophila’s Whole Larvae and Tissues
Maria Cristina OnoratiLuca Lo PiccoloRosa Bonaccorsosubject
Cytoplasmanimal structuresved/biology.organism_classification_rank.speciesBiologyCell FractionationCatalysislcsh:ChemistryInorganic ChemistryCytoplasmic proteinBotanyTechnical NoteAnimalsDrosophila ProteinsPhysical and Theoretical ChemistryModel organismlcsh:QH301-705.5Molecular BiologyDrosophilaSpectroscopyCell NucleusLarvaved/biologyOrganic ChemistryExtraction (chemistry)fungiproteins extractionEmbryoGeneral Medicinebiology.organism_classificationhnRNPsComputer Science ApplicationsDrosophila melanogasterlcsh:Biology (General)lcsh:QD1-999BiochemistryCytoplasmLarvaDrosophilaFunction (biology)description
The identification and study of protein’s function in several model organisms is carried out using both nuclear and cytoplasmic extracts. For a long time, Drosophila’s embryos have represented the main source for protein extractions, although in the last year, the importance of collecting proteins extracts also from larval tissues has also been understood. Here we report a very simple protocol, improved by a previously developed method, to produce in a single extraction both highly stable nuclear and cytoplasmic protein extracts from a small quantity of whole Drosophila’s larvae or tissues, suitable for biochemical analyses like co-immunoprecipitation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-06-01 | International Journal of Molecular Sciences |