6533b858fe1ef96bd12b64b4

RESEARCH PRODUCT

Large scale preparation of human MHC class II+ integrin beta(1)+ Tregs.

Helmut JonuleitJohn WijdenesMagdalena Swiatek-de LangeFranz-joseph SchneiderMartin LenterBrigitte BonninChristian BeckerAnita Correll

subject

Regulatory T cellImmunologyPopulationIntegrinchemical and pharmacologic phenomenaIntegrin alpha4beta1T-Lymphocytes RegulatoryT-Lymphocyte SubsetsmedicineImmune ToleranceImmunology and AllergyHumansIL-2 receptorLeukapheresiseducationCells CulturedMHC class IIeducation.field_of_studybiologyImmunomagnetic SeparationHistocompatibility Antigens Class IIInterleukin-2 Receptor alpha SubunitFOXP3hemic and immune systemsForkhead Transcription FactorsT lymphocyteMHC restrictionFlow CytometryCell biologyHigh-Throughput Screening Assaysmedicine.anatomical_structureImmunologyCD4 Antigensbiology.protein

description

Abstract The human CD4 + CD25 + FoxP3 + regulatory T cell population (Tregs) contains both MHC class II + and MHC class II − cells. MHC class II + Tregs belong to the integrin α 4 β 1 + subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isolation of these MHC class II expressing Tregs from large leukaphereses products using magnetic microbeads that achieves a reproducible purity of more than 90% and enables the use of this small-sized Treg population in pre-clinical application and basic research.

yearjournalcountryeditionlanguage
2010-04-06Journal of immunological methods
10.1016/j.jim.2010.06.014https://pubmed.ncbi.nlm.nih.gov/20599542