6533b858fe1ef96bd12b64e5

RESEARCH PRODUCT

FIA-fluorimetric determination of thiamine.

J. Martínez CalatayudC. Gómez BenitoD.gaspar Gimenez

subject

VitaminAnalyteAqueous solutionChromatographyChemistryPotassiumClinical BiochemistryPharmaceutical Sciencechemistry.chemical_elementPharmaceutical formulationFluorescence spectroscopyAnalytical Chemistrychemistry.chemical_compoundBasic solutionDrug DiscoveryThiamineFluorometryIndicators and ReagentsThiamineOxidation-ReductionSpectroscopyResins Plant

description

A flow injection-fluorimetric determination of thiamine is reported. The procedure is based on the oxidation of the analyte with potassium hexacyanoferrate(III) immobilized on an anionic exchange resin; the fluorescence is monitored in aqueous basic solution. Concentrations of the vitamin of 0.1-4 ppm have been determined; the relative standard deviation was 1.8%. The injection rate was 28 samples/h. The influence of other substances and the determination of the drug in a pharmaceutical formulation are also reported.

10.1016/0731-7085(90)80099-bhttps://pubmed.ncbi.nlm.nih.gov/2100604