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RESEARCH PRODUCT
FIA-fluorimetric determination of thiamine.
J. Martínez CalatayudC. Gómez BenitoD.gaspar Gimenezsubject
VitaminAnalyteAqueous solutionChromatographyChemistryPotassiumClinical BiochemistryPharmaceutical Sciencechemistry.chemical_elementPharmaceutical formulationFluorescence spectroscopyAnalytical Chemistrychemistry.chemical_compoundBasic solutionDrug DiscoveryThiamineFluorometryIndicators and ReagentsThiamineOxidation-ReductionSpectroscopyResins Plantdescription
A flow injection-fluorimetric determination of thiamine is reported. The procedure is based on the oxidation of the analyte with potassium hexacyanoferrate(III) immobilized on an anionic exchange resin; the fluorescence is monitored in aqueous basic solution. Concentrations of the vitamin of 0.1-4 ppm have been determined; the relative standard deviation was 1.8%. The injection rate was 28 samples/h. The influence of other substances and the determination of the drug in a pharmaceutical formulation are also reported.
year | journal | country | edition | language |
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1990-01-01 | Journal of pharmaceutical and biomedical analysis |