6533b858fe1ef96bd12b6b8e
RESEARCH PRODUCT
Evaluation of the proteolysis degree with the o-phthalaldehyde/N-acetyl-L-cysteine reagent
M.j.medina HernándezM.c.garcía Alvarez-coqueR. M. Villanueva CamañasE. Bonet Domingosubject
ChymotrypsinChromatographybiologymedicine.diagnostic_testChemistryProteolysisPronaseTrypsinBiochemistryAnalytical ChemistryO-Phthalaldehydechemistry.chemical_compoundReagentbiology.proteinmedicineLysozymeBovine serum albuminmedicine.drugdescription
The o-phthalaldehyde/N-acetyl-L-cysteine (OPA-NAC) reagent is applied to the spectrophotometric evaluation of the proteolytic activity of enzymes. The high stability of the OPA-NAC isoindoles makes a strict control of the time of reaction unnecessary. A mathematical expression is proposed to calculate proteolysis degrees, where the absorbance decrease of the OPA-NAC derivative of the protein itself during the hydrolysis process is taken into account. The method is applied to bovine serum albumine, caseine, lysozyme, lactoglobuline and protamine sulphate as substrates, and pronase, papaine, trypsin and chymotrypsin as enzymes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-01-01 | Fresenius' Journal of Analytical Chemistry |