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RESEARCH PRODUCT
Pressurized flow electrochromatography with reversed phase capillary columns
Klaus K. UngerThomas EimerTakao Tsudasubject
Capillary electrochromatographyChromatographyCapillary electrophoresisElectrochromatographyChemistryCapillary actionPhase (matter)Analytical chemistryReversed-phase chromatographyBiochemistryCapacity factorPressure gradientAnalytical Chemistrydescription
Pressurized flow electrochromatography (PEC) is a hybrid of capillary LC and capillary electroendosmotic chromatography (CEC). Both a pressure gradient and an electric field are applied across a packed capillary. The feasability of a simple, easy to handle PEC instrumentation is demonstrated. Home made capillary columns with four different silica-based reversed phase packings have been operated under PEC conditions separating non ionic and ionic low molecular weight analytes. The capillary columns have been characterized with respect to their separation efficiency and selectivity and the results have been compared to those obtained with the purely pressure driven system. An electrochromatographic capacity factor is discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-01-01 | Fresenius' Journal of Analytical Chemistry |