0000000000341474
AUTHOR
Thomas Eimer
Selectivity tuning in pressurized-flow electrochromatography
Abstract Pressurized-flow electrochromatography (PEC) is a developing separation technique in which both a pressure gradient and an electric field are applied across a packed capillary. In this work we present new results illustrating the principles and the potential of PEC. Home-made capillary columns with silica-based reversed phase packings were operated under PEC conditions separating low molecular weight analytes. Compared to the purely pressure-driven system enhanced selectivity for the charged analytes was observed. It is shown that the retention time of a retained cationic analyte in PEC can be calculated using the chromatographic capacity factor and the electrophoretic mobility of …
Pressurized flow electrochromatography with reversed phase capillary columns
Pressurized flow electrochromatography (PEC) is a hybrid of capillary LC and capillary electroendosmotic chromatography (CEC). Both a pressure gradient and an electric field are applied across a packed capillary. The feasability of a simple, easy to handle PEC instrumentation is demonstrated. Home made capillary columns with four different silica-based reversed phase packings have been operated under PEC conditions separating non ionic and ionic low molecular weight analytes. The capillary columns have been characterized with respect to their separation efficiency and selectivity and the results have been compared to those obtained with the purely pressure driven system. An electrochromatog…