6533b859fe1ef96bd12b6f3a
RESEARCH PRODUCT
Human macrophages simultaneously express membrane-C1q and Fc-receptors for IgG
Marcus KaulMarcus KaulMichael LoosStephan StörkelPatrick Terheydensubject
ImmunoprecipitationCD14ImmunologyReceptors FcBiologyFlow cytometrymedicineFluorescence microscopeHumansImmunoprecipitationImmunology and AllergyNorthern blotReceptorCells Culturedmedicine.diagnostic_testComplement C1qMacrophagesCell MembraneCell DifferentiationMolecular biologyIn vitroCell biologyBlotGene Expression RegulationImmunoglobulin GProtein Bindingdescription
Membrane C1q (mC1q) of macrophages (MPhi) is a precursor of the IgG-binding serum protein C1q. Thus, mC1q potentially provides one of several Fcgamma binding sites of mature MPhi and we analyzed whether simultaneous expression occurs of established receptors for IgG, FcgammaRI, II, and III, and mC1q during in vitro differentiation of MPhi. Using flow cytometry, immunoprecipitation combined with Western blotting and Northern blot analysis mC1q was hardly detected in freshly isolated blood monocytes, but increasingly in developing monocyte-derived MPhi. Laser scanning fluorescence microscopy confirmed the membrane localization of mC1q. Two-color-staining flow cytometry experiments indicated that mC1q and all three types of FcgammaRs are simultaneously expressed on mature monocyte-derived MPhi. A high correlation was found for the expression of mC1q and FcgammaRs, in particular FcgammaRII, but not mC1q and CD14, another marker of monocytes/MPhi.
year | journal | country | edition | language |
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2005-11-01 | Immunology Letters |