6533b859fe1ef96bd12b6f5b

RESEARCH PRODUCT

Computer-aided calculation of the molecular size of nondenatured proteins in pore-gradient gel electrophoresis

Gunter M. RotheHans Weidmann

subject

Gel electrophoresisComputer programChemistryClinical BiochemistryAnalytical chemistryProteinsCharacter encodingRadiusFunction (mathematics)Table (information)BiochemistryDisk operating systemAnalytical ChemistryComputational scienceMolecular WeightModels ChemicalComputer-aidedElectrophoresis Polyacrylamide GelMathematicsSoftware

description

A computer program written in Turbo C is described, which uses the two-step mathematical procedure published recently (Rothe, G. M., Electrophoresis 1988, 9, 307-316) to evaluate the molecular mass, Stokes' radius, spherical radius, and frictional coefficient of nondenatured proteins. The program runs on any IBM-PC or 100% compatible IBM-PC, provided the disk operating system MS-DOS or PC-DOS 3.0 or later has been installed. Functions that are permanently in use are accessible by menu. Storage and loading of data from disk and help instructions can be called by use of function keys. The program provides several tables into which inserted and calculated data is automatically integrated. Each table can be printed out, provided a printer with IBM character set is connected to the computer.

https://doi.org/10.1002/elps.1150121004