Ekstracelulārās vezikulās ietvertās mikrobiālās RNS atklāšana cilvēku asins un urīna paraugos

6533b859fe1ef96bd12b71ec

RESEARCH PRODUCT

Ekstracelulārās vezikulās ietvertās mikrobiālās RNS atklāšana cilvēku asins un urīna paraugos

Anastasija Rudņickiha

subject

bacterial transcriptome cell – free RNA Bioloģija extracellular vesicles outer membrane vesicles

description

Gandrīz visi cilvēka šūnu tipi sekretē ekstracelulārās vezikulas (EVs), un tām ir svarīga loma starpšūnu komunikācijā. Jaunākie pētījumi liecina, ka komunikācija notiek arī starp cilvēka mikrobiotu un saimniekorganisma šūnām. Šajā pētījumā mēs parādām, ka no cilvēka asinīm un urīna izdalītās EVs satur RNS fragmentus no dažādiem mikroorganismiem. Visbiežāk sastopami baktēriju tipi un supertipi asinīs un urīnā bija Proteobacteria, PVC grupa un Terrabacteria. Lai noteiktu vai šo mikrobiālo transkriptu galvenais avots ir zarnu mikrobioms, mēs izstrādājām EV izdalīšanas metodi no cilvēku fēču paraugiem. Tālāk mēs plānojām veikt RNS sekvencēšanas (RNA-seq) analīzi EV, kas izdalītas no plazmas, urīna un fēcēm no tiem pašiem indivīdiem.

year	journal	country	edition	language
2020-01-01

https://dspace.lu.lv/dspace/handle/7/51667

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