6533b859fe1ef96bd12b756c

RESEARCH PRODUCT

MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells

Edward A. LemkeEdward A. LemkeEdward A. LemkeChristine KoehlerChristine KoehlerChristine Koehler

subject

0301 basic medicinechemistry.chemical_classificationbiologyChemistryvirusesBaculovirus expressionComputational biologySpodopteraGenetic codebiology.organism_classificationAmino acidTransposition (music)03 medical and health sciences030104 developmental biologyTransfer RNAGeneSf21

description

The combination of genetic code expansion (GCE) and baculovirus-based protein expression in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes with site-specifically introduced noncanonical amino acids. This protocol describes the integration of synthetase and tRNA gene indispensable for GCE into the backbone of the Bacmid, the Tn7-mediated transposition of various genes of interest, as well as the final expression of protein using the MultiBacTAG system with different noncanonical amino acids.

yearjournalcountryeditionlanguage
2018-01-01
https://doi.org/10.1007/978-1-4939-7574-7_19