6533b859fe1ef96bd12b7597

RESEARCH PRODUCT

Genetic diversity ofNarcissus tortifolius, an endangered endemic species from Southeastern Spain

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AbstractIn this work, we analyzed inter-simple sequence repeat markers from 10 populations (298 individuals) spanning the whole distribution range of the endemic Narcissus tortifolius. We assessed genetic variation levels and distribution by estimates of genetic diversity, analysis of molecular variance (AMOVA), principal coordinates and Bayesian methods. N. tortifolius showed moderate genetic diversity at intrapopulation level and low genetic differentiation of populations. In general, Almerian populations showed slightly higher levels of genetic diversity than Murcian populations. Our results indicate that habitat fragmentation has not caused genetic depauperation in N. tortifolius but did reveal moderate genetic differentiation. Indeed, principal coordinate analysis and Mantel test revealed a slight tendency to separate populations into two groups (Murcian vs. Almerian). A recent isolation event of populations, together with the perennial nature of this species could be the main reasons for this low to...