6533b859fe1ef96bd12b7699
RESEARCH PRODUCT
Improving extracellular vesicles visualization: From static to motion
Muthukumar GunasekaranMarzia PucciPeter VerstraelenChristian RolfoChristian RolfoChristian RolfoSimona TavernaSara BalsRiccardo AlessandroDiego De Miguel-pérezSunjay KaushalPablo ReclusaIsabel PintelonNathalie Claessubject
InteractionIntravital MicroscopyComputer sciencemedia_common.quotation_subjectlcsh:MedicineAntineoplastic AgentsvideoExosomesNSCLCTime-Lapse ImagingExtracellular vesiclesArticleFluorescence imagingExtracellular VesiclesSettore BIO/13 - Biologia ApplicataCell Line TumorNeoplasmsmedicineHumansTissue specificInternalizationlcsh:ScienceBiologymedia_commonDrug CarriersMicroscopy ConfocalMultivesicular bodiesMultidisciplinaryDisease progressionlcsh:RCancerEpithelial Cellsmedicine.diseaseCancer treatmentCell biologyinternalizationNucleic acidsConfocal microscopyTransportersDrug deliveryDisease ProgressionMicroscopy Electron ScanningIsolation separation and purificationlcsh:QHuman medicineextracellular vesicleEngineering sciences. TechnologyUltracentrifugationdescription
AbstractIn the last decade, extracellular vesicles (EVs) have become a hot topic. The findings on EVs content and effects have made them a major field of interest in cancer research. EVs, are able to be internalized through integrins expressed in parental cells, in a tissue specific manner, as a key step of cancer progression and pre-metastatic niche formation. However, this specificity might lead to new opportunities in cancer treatment by using EVs as devices for drug delivery. For future applications of EVs in cancer, improved protocols and methods for EVs isolation and visualization are required. Our group has put efforts on developing a protocol able to track the EVs for in vivo internalization analysis. We showed, for the first time, the videos of labeled EVs uptake by living lung cancer cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-01-01 |