6533b859fe1ef96bd12b7730

RESEARCH PRODUCT

CRP-induced levels of oxidative stress are higher in brain than aortic endothelial cells

Bianca BenderHeiko J. LuhmannDorothea CloshenChristoph R.w. Kuhlmann

subject

medicine.medical_specialtyImmunologyBlood–brain barriermedicine.disease_causeBiochemistryInternal medicinemedicineAnimalsImmunology and AllergyReceptorMolecular BiologyAortachemistry.chemical_classificationReactive oxygen speciesNADPH oxidasebiologyChemistryReceptors IgGBrainEndothelial CellsNADPH OxidasesHematologyOxidative StressC-Reactive Proteinmedicine.anatomical_structureEndocrinologyNAD(P)H oxidaseImmunologybiology.proteinCattleP22phoxNAD+ kinaseOxidative stress

description

C-reactive protein (CRP) has been demonstrated to induce blood-brain barrier disruption (BBB) involving NAD(P)H-oxidase dependent oxidative stress. It is unclear why CRP affects the BBB and not other vascular beds following stroke. Therefore we examined CRP receptor and NAD(P)H-oxidase expression levels in bovine brain- (BEC) and aortic endothelial cells. Dichlorodihydrofluorescein measurements revealed significantly higher CRP-induced reactive oxygen species (ROS) levels in BEC. Protein expression of the CRP-receptors CD16, CD32 and of the NAD(P)H-oxidase subunit p22phox were also significantly higher in BEC. In conclusion BEC show a higher vulnerability to CRP due to increased levels of CRP receptors and the NAD(P)H-oxidase.

yearjournalcountryeditionlanguage
2010-05-01Cytokine
https://doi.org/10.1016/j.cyto.2010.02.011