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RESEARCH PRODUCT
Obesity impairs leukocyte‐endothelium cell interactions and oxidative stress in humans
Carlos MorillasMilagros RochaSilvia VesesIrene Escribano-lopezAntonio Hernández-mijaresCelia BañulsÁNgeles ÁLvarezSandra López-domènechSamuel OrdenVictor M. VictorNoelia Diaz-moralessubject
0301 basic medicineAdultMalemedicine.medical_specialtyEndotheliumAdolescentClinical BiochemistryCell Communication030204 cardiovascular system & hematologyMitochondrionmedicine.disease_causeBiochemistryProinflammatory cytokineBody Mass Index03 medical and health sciencesYoung Adult0302 clinical medicineInternal medicinemedicineCell AdhesionLeukocytesHumansObesityEndothelial dysfunctionCell adhesionAgedchemistry.chemical_classificationMembrane Potential MitochondrialReactive oxygen speciesChemistryCell adhesion moleculeEndothelial CellsGeneral MedicineMiddle AgedAtherogenesismedicine.diseaseIntercellular Adhesion Molecule-1Oxidative Stress030104 developmental biologyEndocrinologymedicine.anatomical_structureCross-Sectional StudiesCytokinesReactive oxygen specieFemaleMitochondrial membrane potentialReactive Oxygen SpeciesOxidative stressdescription
Background:To evaluate the relationship between leukocyte‐endothelial cellinteractions and oxidative stress parameters in non‐diabetic patients with differentgrades of obesity.Material and methods:For this cross‐sectional study, 225 subjects wererecruited from January 1, 2014 to December 31, 2016 and divided into groupsaccording to BMI (40 kg/m²). We determined clin-ical parameters, systemic inflammatory markers, soluble cellular adhesion mole-cules, leukocyte‐endothelium cell interactions—rolling flux, velocity and adhesion—, oxidative stress parameters—total ROS, total superoxide, glutathione—andmitochondrial membrane potential in leukocytes.Results:We verified that HOMA‐IR and hsCRP increased progressively as obe-sity developed, whereas A1c, IL6 and TNFαwere augmented in the BMI > 40kg/m² group. The cellular adhesion molecule sP‐selectin was increased in patientswith obesity, while sICAM, total ROS, total superoxide and mitochondrial mem-brane potential were selectively higher in the BMI > 40 kg/m² group. Obesityinduced a progressive decrease in rolling velocity and an enhancement of rollingflux and leukocyte adhesion.Conclusion:Our findings reveal that endothelial dysfunction markers are alteredin human obesity and are associated with proinflammatory cytokines andincreased oxidative stress parameters.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-08-01 |