6533b85afe1ef96bd12b8ba6

RESEARCH PRODUCT

Nuclear rDNA-based molecular clock of the evolution of Triatominae (Hemiptera : Reduviidae), vectors of Chagas disease

BarguesChristopher J. SchofieldJanine M. RamseySantiago Mas-comaJean-pierre DujardinAntonio Marcilla

subject

Microbiology (medical)Chagas diseaseChagas diseaselcsh:Arctic medicine. Tropical medicinelcsh:RC955-962lcsh:QR1-502ZoologyTAXONOMIEDNA RibosomalPolymerase Chain Reactionnuclear rDNAlcsh:Microbiology18S geneEvolution MolecularBiological ClocksevolutionRNA Ribosomal 18SmedicineETUDE COMPARATIVEAnimalsINSECTE NUISIBLECell LineagePHYLOGENIEInternal transcribed spacerMolecular clockRibosomal DNATriatominaeHEURE MOLECULAIRETriatominae vectorsGENE 18SBase SequencebiologyVECTEURITS2 SPACER.INTERNAL TRANSCRIBED SPACERmolecular clockSequence Analysis DNAbiology.organism_classificationmedicine.diseaseHemipteraEVOLUTIONInsect VectorsReduviidaeMALADIE DE CHAGASTaxonomy (biology)TriatominaeITS-2 spacerANALYSE GENETIQUE

description

The evolutionary history and times of divergence of triatomine bug lineages are estimated from molecular clocks inferred from nucleotide sequences of the small subunit SSU (18S) and the second internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA of these reduviids. The 18S rDNA molecular clock rate in Triatominae, and Prosorrhynchan Hemiptera in general, appears to be of 1.8% per 100 million years (my). The ITS-2 molecular clock rate in Triatominae is estimated to be around 0.4-1% per 1 my, indicating that ITS-2 evolves 23-55 times faster than 18S rDNA. Inferred chronological data about the evolution of Triatominae fit well with current hypotheses on their evolutionary histories, but suggest reconsideration of the current taxonomy of North America species complexes. (Résumé d'auteur)

yearjournalcountryeditionlanguage
2000-08-01
http://www.documentation.ird.fr/hor/fdi:010022772