6533b85afe1ef96bd12b8bfe

RESEARCH PRODUCT

Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching

Maxim MayzelB. Göran KarlssonUlrika BrathLinnéa IsakssonCecilia PerssonVladislav Yu. OrekhovJanne A. IhalainenSebastian WestenhoffLidija VrhovacEmil Gustavsson

subject

Models MolecularLightTongue regionBiophysicsphototransduction03 medical and health sciences0302 clinical medicineProtein DomainsPHYmolekyylidynamiikkaprotein structureNMR-spektroskopiaNuclear Magnetic Resonance Biomolecular030304 developmental biologyphytochrome0303 health sciencesPhytochromebiologyChemistryProtein NMR SpectroscopyDeinococcus radioduransArticlesDarknessbiology.organism_classificationmolecular dynamicsNMRStructural heterogeneityDark stateModulationBiophysicsvalokemiaproteiinitDeinococcusPhytochrome030217 neurology & neurosurgery

description

Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although crystal structures of dark and light adapted states have been determined, the molecular mechanisms underlying photoactivation remains elusive. Here we demonstrate that the conserved tongue region of the PHY domain of a 57kDa photosensory module of Deinococcus radiodurans phytochrome, changes from a structurally heterogeneous dark state to an ordered light activated state. The results were obtained in solution by utilizing a laser-triggered activation approach detected on the atomic level with high-resolution protein NMR spectroscopy. The data suggest that photosignaling of phytochromes relies on careful modulation of structural heterogeneity of the PHY tongue. peerReviewed

yearjournalcountryeditionlanguage
2019-11-26Biophysical Journal
https://doi.org/10.1016/j.bpj.2019.11.025