6533b85afe1ef96bd12b94ba
RESEARCH PRODUCT
Structural alterations of faecal and mucosa-associated bacterial communities in irritable bowel syndrome
Marta PonceVicente GarriguesJulio PonceAna DurbánAndrés MoyaNuria Jiménez-hernándezPatricia SalgadoAmparo LatorreJ.j. Abellánsubject
medicine.medical_specialtyBiologymedicine.diseaseAgricultural and Biological Sciences (miscellaneous)GastroenterologyMicrobiologyDescending colonmedicine.anatomical_structureFunctional gastrointestinal disorderInternal medicinemedicineEcology Evolution Behavior and SystematicsIrritable bowel syndromeFecesMicrobiota compositiondescription
Summary Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder in western coun- tries. Previous studies on IBS, mostly based on faecal samples, suggest alterations in the intestinal micro- biota. However, no consensus has been reached regarding the association between specific bacteria and IBS. We explore the alterations of intestinal bac- terial communities in IBS using massive sequencing of amplified 16S rRNA genes. Mucosal biopsies of the ascending and descending colon and faeces from 16 IBS patients and 9 healthy controls were analysed. Strong inter-individual variation was observed in the composition of the bacterial communities in both patients and controls. These communities showed less diversity in IBS cases. There were larger differ- ences in the microbiota composition between biop- sies and faeces than between patients and controls. We found a few over-represented and under-
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-03-01 | Environmental Microbiology Reports |