6533b85afe1ef96bd12b9825
RESEARCH PRODUCT
Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas.
Ingrid GarzónIngrid GarzónJesus Chato-astrainJesus Chato-astrainCarmen González-gallardoAna IonescuJuan De La Cruz CardonaMiguel MateuMiguel MateuCarmen CardaMaría Del Mar PérezMiguel ÁNgel Martín-piedraMiguel ÁNgel Martín-piedraMiguel AlaminosMiguel Alaminossubject
0301 basic medicinePathology02 engineering and technology:Chemicals and Drugs::Carbohydrates::Polysaccharides::Sepharose [Medical Subject Headings]Umbilical cord:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]heterotypical human corneaTissue engineering:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Lagomorpha::Rabbits [Medical Subject Headings]Cornea:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Optical Imaging::Tomography Optical::Tomography Optical Coherence [Medical Subject Headings]:Organisms::Eukaryota::Animals [Medical Subject Headings]:Technology and Food and Beverages::Technology Industry and Agriculture::Manufactured Materials::Biomedical and Dental Materials::Biocompatible Materials [Medical Subject Headings]Slit lamp021001 nanoscience & nanotechnologymedicine.anatomical_structure:Anatomy::Sense Organs::Eye::Anterior Eye Segment::Cornea [Medical Subject Headings]tissue engineeringStem cell0210 nano-technologyBiotechnology:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Blood Proteins::Fibrin [Medical Subject Headings]medicine.medical_specialtyHistologyStromal celllcsh:BiotechnologyBiomedical EngineeringCélulas madre mesenquimatosasBioengineering:Anatomy::Embryonic Structures::Fetus::Umbilical Cord [Medical Subject Headings]:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models Animal [Medical Subject Headings]03 medical and health sciencesIn vivolcsh:TP248.13-248.65medicine:Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells [Medical Subject Headings]:Technology and Food and Beverages::Technology Industry and Agriculture::Engineering::Bioengineering::Cell Engineering::Tissue Engineering [Medical Subject Headings]Wharton’s jelly stem cellsbioengineered corneabusiness.industryTissue engineringeye diseasesEpitheliumCórnea:Anatomy::Cells::Epithelial Cells [Medical Subject Headings]:Anatomy::Tissues::Connective Tissue::Wharton Jelly [Medical Subject Headings]030104 developmental biologyIngeniería de tejidossense organsbusinessartificial corneadescription
Purpose: Human cornea substitutes generated by tissue engineering currently require limbal stem cells for the generation of orthotypical epithelial cell cultures. We recently reported that bioengineered corneas can be fabricated in vitro from a heterotypical source obtained from Wharton’s jelly in the human umbilical cord (HWJSC). Methods: Here, we generated a partial thickness cornea model based on plastic compression nanostructured fibrin-agarose biomaterials with cornea epithelial cells on top, as an orthotypical model (HOC), or with HWJSC, as a heterotypical model (HHC), and determined their potential in vivo usefulness by implantation in an animal model. Results: No major side effects were seen 3 and 12 months after implantation of either bioengineered partial cornea model in rabbit corneas. Clinical results determined by slit lamp and optical coherence tomography were positive after 12 months. Histological and immunohistochemical findings demonstrated that in vitro HOC and HHC had moderate levels of stromal and epithelial cell marker expression, whereas in vivo grafted corneas were more similar to control corneas. Conclusion: These results suggest that both models are potentially useful to treat diseases requiring anterior cornea replacement, and that HHC may be an efficient alternative to the use of HOC which circumvents the need to generate cornea epithelial cell cultures.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-06-19 |