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RESEARCH PRODUCT
Effects of Citrus Exocortis Viroid Infection on the Peroxidase/IAA-Oxidase System of Gynura aurantiaca and Lycoperszcon esculentum
F. FloresJ.-l. Rodriguezsubject
Oxidase testbiologyViroidInoculationfood and beveragesGeneral MedicineGeneral Chemistrybiology.organism_classificationIsozymeLycopersiconBiochemistrybiology.proteinGynura aurantiacaPolyacrylamide gel electrophoresisPeroxidasedescription
Summary The peroxidase/indoleacetic acid (IAA) oxidase system of plants of Gynura aurantiaca and Lycopersicon esculentum healthy and infected by citrus exocortis viroid (CEV) was studied. In these hosts, the infection induced an increase of both enzymic activities, when they were measured between 40 and 50 d post inoculation. The analysis of isozyme composition by polyacrylamide gel electrophoresis and specific staining of peroxidase revealed an intensification of most of the zymogram bands as well as the premature appearance of one of the isozymes in the two plants investigated. All the tomato isoperoxidases exhibited IAA-oxidase activity when measured with the oxygen electrode. These results could procide an explanation for the lower amounts of a diffusible substance with activity and RF similar to IAA, as well as for some other alterations found in CEV-infected plants. Moreover, the close resemblance of the changes in the isozyme pattern, suggests that in both plants the mechanism of viroid pathogenesis may occur through some common steps.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-01-01 | Biochemie und Physiologie der Pflanzen |