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RESEARCH PRODUCT
Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function.
Jürgen KnopJohn WijdenesPetra LutterChristian BeckerTobias BoppSabine StollEva HuterChristoph RichterHansjörg SchildJan KubachPetra WeingartenStefan MüllnerTobias WargerEdgar SchmittHelmut Jonuleitsubject
ProteomeGalectinsImmunologychemical and pharmacologic phenomenaBiologyBiochemistryT-Lymphocytes RegulatoryFlow cytometrymedicineHumansIL-2 receptorCells CulturedGalectinCell ProliferationClonal AnergyMessenger RNAmedicine.diagnostic_testFOXP3Antibodies Monoclonalhemic and immune systemsForkhead Transcription FactorsCell BiologyHematologyCell biologySelf ToleranceGene Expression RegulationProteomeImmunologyIntracellularFunction (biology)Biomarkersdescription
AbstractCD4+CD25+Foxp3+ regulatory T cells (CD25+ Treg cells) direct the maintenance of immunological self-tolerance by active suppression of autoaggressive T-cell populations. However, the molecules mediating the anergic state and regulatory function of CD25+ Treg cells are still elusive. Using differential proteomics, we identified galectin-10, a member of the lectin family, as constitutively expressed in human CD25+ Treg cells, while they are nearly absent in resting and activated CD4+ T cells. These data were confirmed on the mRNA and protein levels. Single-cell staining and flow cytometry showed a strictly intracellular expression of galectin-10 in CD25+ Treg cells. Specific inhibition of galectin-10 restored the proliferative capacity of CD25+ Treg cells and abrogated their suppressive function. Notably, first identified here as expressed in human T lymphocytes, galectin-10 is essential for the functional properties of CD25+ Treg cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-05-16 | Blood |