6533b85bfe1ef96bd12ba2e0

RESEARCH PRODUCT

Alkaline hydrolysis/methylation-acetylation: a new technique for ultrastructural DNA cytochemistry.

J. M. FerrerA. R. LlorenteA. TatoJuan C. StockertJ. B. RomeroP. Del CastilloC. J. Tandler

subject

HistologyHydrolysisUranyl acetateAcetylationRats Inbred StrainsDNABiologyAlkaline hydrolysis (body disposal)Molecular biologyMethylationChromatinPathology and Forensic MedicineChromatinStainingRatschemistry.chemical_compoundMicroscopy ElectronchemistryBiochemistryBone MarrowUltrastructureNucleic acidCytochemistryAnimalsDNA

description

SUMMARY A new technique for the visualization of DNA-containing structures in electron microscopy is described. Samples of glutaraldehyde-fixed bone marrow from rats were subjected to alkaline hydrolysis to remove RNA and the phosphate of phospho-proteins, followed by a combined blockage of protein carboxyl and amino groups through methylation-acetylation. After uranyl acetate staining of epoxy-embedded ultrathin sections, chromatin from all cell types showed a highly selective and intense electron opacity. Staining methods for DNA were also positive in semithin sections. This simple procedure could be very useful in ultrastructural cytochemistry of DNA and chromatin.

yearjournalcountryeditionlanguage
1991-05-01Journal of microscopy
10.1111/j.1365-2818.1991.tb03139.xhttps://pubmed.ncbi.nlm.nih.gov/1865496