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RESEARCH PRODUCT
dsDNA, ssDNA, G-quadruplex DNA, and nucleosomal DNA electrochemical screening using canthin-6-one alkaloid-modified electrodes
Antonio Doménech-carbóGerardo Cebrián-torrejónGerardo Cebrián-torrejónDaniel Rodrigues-furtadoErwan PouponBruno FigadèreVicente TorderaAlain FournetLaura De MiguelSuzana Assad-kahnRafael P. Vázquez-manriquesubject
Detection limitChemistryGeneral Chemical EngineeringIntercalation (chemistry)Glassy carbonG-quadruplexElectrochemistrychemistry.chemical_compoundScanning electrochemical microscopyBiochemistryDNA methylationElectrochemistryBiophysicsDNAdescription
Abstract Microparticulate films of a canthin-6-one alkaloid ( L ), a natural β-carboline alkaloid presenting a characteristic naphtyridone motif, on glassy carbon electrodes yield different, separate voltammetric signals for dsDNA, ssDNA, G-quadruplex DNA, different degrees of DNA methylation and the biomimetic nucleosomal DNA with detection limit of 10 −5 M. This multiple-signal electrochemical behavior is in contrast with conventional use of DNA intercalators, only discriminating between different DNA forms by variations in the intensity of a unique signal. Complementary photochemical and scanning electrochemical microscopy (SECM) data suggest that the differences in the voltammetric response of the different DNA forms derive from the intercalating properties of the canthin-6-one.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 | Electrochimica Acta |