IP 3 signalling regulates exogenous RNA i in C aenorhabditis elegans
RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology…
Synergistic activation of AMPK prevents from polyglutamine-inducedtoxicity inCaenorhabditis elegans
11 páginas, 4 figuras. Supplementary material related to this article can be found, in the online version, at doi: https://doi.org/10.1016/j.phrs.2020.105105.
dsDNA, ssDNA, G-quadruplex DNA, and nucleosomal DNA electrochemical screening using canthin-6-one alkaloid-modified electrodes
Abstract Microparticulate films of a canthin-6-one alkaloid ( L ), a natural β-carboline alkaloid presenting a characteristic naphtyridone motif, on glassy carbon electrodes yield different, separate voltammetric signals for dsDNA, ssDNA, G-quadruplex DNA, different degrees of DNA methylation and the biomimetic nucleosomal DNA with detection limit of 10 −5 M. This multiple-signal electrochemical behavior is in contrast with conventional use of DNA intercalators, only discriminating between different DNA forms by variations in the intensity of a unique signal. Complementary photochemical and scanning electrochemical microscopy (SECM) data suggest that the differences in the voltammetric res…
Genotype and phenotype analysis of Friedreich's ataxia compound heterozygous patients
Friedreich's ataxia is caused by mutations in the FRDA gene that encodes frataxin, a nuclear-encoded mitochondrial protein. Most patients are homozygous for the expansion of a GAA triplet repeat within the FRDA gene, but a few patients show compound heterozygosity for a point mutation and the GAA-repeat expansion. We analyzed DNA samples from a cohort of 241 patients with autosomal recessive or isolated spinocerebellar ataxia for the GAA triplet expansion. Patients heterozygous for the GAA expansion were screened for point mutations within the FRDA coding region. Molecular analyses included the single-strand conformation polymorphism analysis, direct sequencing, and linkage analysis with FR…