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RESEARCH PRODUCT
IP 3 signalling regulates exogenous RNA i in C aenorhabditis elegans
Marie LopezMaría Dolores SequedoAnna E HerlihyAnikó I. NagyRafael P. Vázquez-manriqueMaxime BodakHoward A. BaylisChristo P. ChristovRoxani GatsiMareike Herzogsubject
inorganic chemicalscalcium signallingCell signalingMutantInositol 145-TrisphosphateBiologyModels BiologicalBiochemistryRNA interferenceRNA interferenceImage Processing Computer-AssistedGeneticsAnimalsIntestinal MucosaCaenorhabditis elegansMolecular BiologyCaenorhabditis elegansRNA Double-StrandedCalcium signalingenhanced RNAiScientific Reportsfungiinositol 145‐trisphosphateRNAbiology.organism_classificationC. elegansCell biologySignallingMicroscopy FluorescenceSignal transductionSignal Transductiondescription
RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology or environment.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-01-21 | EMBO reports |