6533b85bfe1ef96bd12bb423

RESEARCH PRODUCT

Functionalized Polystyrene Nanoparticles Trigger Human Dendritic Cell Maturation Resulting in Enhanced CD4+T Cell Activation

Katharina LandfesterNicole BacherKerstin SteinbrinkVolker MailänderGrit BaierStefanie U. Frick

subject

CD4-Positive T-LymphocytesPolymers and Plasticsmedicine.medical_treatmentT cellOrganophosphonatesNanoparticleBioengineeringLymphocyte ActivationFunctionalized polystyreneBiomaterialsInterferon-gammachemistry.chemical_compoundDownregulation and upregulationMaterials ChemistrymedicineHumansImmunologic FactorsMicroscopy ConfocalCd4 t cellChemistryDendritic CellsImmunotherapyDendritic cellCell biologymedicine.anatomical_structureImmunologyCytokinesNanoparticlesPolystyrenesPolystyreneSulfonic AcidsBiotechnology

description

Nanoparticles (NP) represent a promising tool for biomedical applications. Here, sulfonate- and phosphonate-functionalized polystyrene NP are analyzed for their interaction with human monocyte-derived dendritic cells (DC). Immature dendritic cells (iDC) display a higher time- and dose-dependent uptake of functionalized polystyrene NP compared to mature dendritic cells (mDC). Notably, NP induce an enhanced maturation of iDC but not of mDC (upregulation of stimulatory molecules and cytokines). NP-triggered maturation results in a significantly enhanced T cell stimulatory capacity (increased CD4(+) T cell proliferation and IFN-γ production), indicating a shift to a pronounced Th1 response. Immunomodulatory properties of NP may be a useful strategy for strengthening the efficacy of NP-based approaches in immunotherapy.

yearjournalcountryeditionlanguage
2012-10-05Macromolecular Bioscience
https://doi.org/10.1002/mabi.201200223