6533b85bfe1ef96bd12bb47f

RESEARCH PRODUCT

Lateral organization of G M1 in phase-separated monolayers visualized by scanning force microscopy

Stephanie KünnekeAndreas JanshoffManuela Menke

subject

Aqueous solutionChemistryLipid BilayersBiophysicsAnalytical chemistryBrainMembranes ArtificialG(M1) GangliosideGeneral MedicineMicroscopy Atomic ForceLipidsMicelleSphingomyelinschemistry.chemical_compoundCrystallographyCholesterolGlycolipidPhase (matter)MicroscopyMonolayerPhosphatidylcholinesSphingomyelinPOPC

description

Phase separation of glycolipids in lipid mono- and bilayers is of great interest for the understanding of membrane function. The distribution of the ganglioside GM1 in sphingomyelin (SM)/1-palmitoyl-2-oleoyl- sn-glycero-3-phosphocholine (POPC), SM/1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DOPC) and SM/cholesterol/POPC Langmuir-Blodgett (LB) monolayers transferred at 36 mN/m has been studied by scanning force microscopy. Besides lateral organization of the glycolipid in LB monolayers as deduced from topography, material properties have been investigated by phase imaging, pulsed force mode and force modulation microscopy. It was shown that GM1 preferentially clusters in an ordered lipid matrix, i.e. the SM phase in the case of the SM/POPC and SM/DOPC mixture or in the ordered phase of POPC/SM/cholesterol monolayers. At higher local concentrations, three-dimensional protrusions enriched in GM1 occur, which may represent a precursor for the formation of micelles budding into the aqueous subphase. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00249-002-0232-4.

yearjournalcountryeditionlanguage
2002-01-14European Biophysics Journal
https://doi.org/10.1007/s00249-002-0232-4