6533b85bfe1ef96bd12bb50f
RESEARCH PRODUCT
Marine organisms as source of bioactive molecules applied in restoration projects
G. BarresiMatteo CammarataMaria Rosa TrapaniMaria Giovanna ParisiMaria Francesca MuleFranco PallaEnza Di CarloChiara Chillesubject
ArcheologyBiocideProteaseMarine invertebrate Biocleaning Protein layer Protease Antimicrobial peptides Biodegradation controlbiologymedicine.medical_treatmentMicrococcusBioactive moleculesConservationBacterial growthAspergillus sojaebiology.organism_classificationAntimicrobialMinimum inhibitory concentrationMarine organismPenicilliumSettore BIO/03 - Botanica Ambientale E ApplicatamedicineFood sciencedescription
Abstract In recent decades research in the conservation and restoration field has provided sustainable alternatives to traditional procedures for cleaning or controlling the microbial colonization of works of art. In the present study, for the first time novel bioactive molecules extracted from marine invertebrate organisms (Anthozoa) were tested instead of chemical compounds for removing protein layers or as a biocide for controlling fungal or bacterial colonization. In particular, Bioactive Molecules with Protease activity (BMP), acting in a temperature range of 4- 30°C, were tested for the hydrolysis of protein layers on laboratory specimens. The cleaning protocol provides a selective procedure to avoid damage to the original materials constituting the heritage object. Concurrently, enzymatic cleaning was also performed using commercial Protease from Aspergillus sojae (Type XIX), in order to compare their hydrolytic activities. Bioactive Molecules with Antimicrobial activity (BMA1, BMA2) were tested to control bacterial (Bacillus, Micrococcus) or fungal (Aspergillus, Penicillium) growth, previously isolated from colonized canvas samples and characterized by an integrated approach based on in vitro culture, microscopy and molecular investigations. These molecules were tested to define the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal/ Fungicidal Concentration (MBC/MFC). Specifically, BMAs were used to control fungal growth during the relining of the painting (laboratory specimens), carried out using a canvas support, and glue paste as binder. In our hypothesis, these molecules provide an important contribution to the development of innovative protocols for biocleaning or microbial growth control, based on fast and easy application, operator friendly and environmentally sustainable molecules.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-05-25 |