6533b85bfe1ef96bd12bb70c
RESEARCH PRODUCT
Characterization of a proton pump from Acer pseudoplatanus cell microsomes
M.r. AllardR. ScallaJean-pierre BleinL. Sutysubject
0106 biological sciencesOligomycinATPaseDiaphragm pumpPlant ScienceBiology01 natural sciences[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health scienceschemistry.chemical_compound[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsProton transportGenetics030304 developmental biologychemistry.chemical_classification0303 health sciencesAcridine orangeERABLE FAUX PLATANEGeneral MedicinePOMPE PROTONAcer pseudoplatanusbiology.organism_classificationEnzymeBiochemistrychemistrybiology.proteinMicrosomeAgronomy and Crop Science010606 plant biology & botanydescription
Abstract An Acer pseudoplatanus cell microsomal fraction was enriched in ATPase by sedimentation through a sucrose cushion and treatment with Triton X-100. This activity, which reached 0.9 μmol P i min −1 mg −1 protein, was specific for ATP, slightly stimulated by K + , inhibited by orthovanadate and diethylstilbestrol, insensitive to oligomycin and azide, and had a K m - value of 0.51 mM for MgATP. ATP-dependent proton translocation was demonstrated by the ΔpH probe acridine orange. This activity had a optimum at pH 6.5, was substrate specific for ATP, and was strongly dependent on K + . Preparations of plasma membrane ATPase from A. pseudoplatanus cell culture thus posses biochemical properties analogous to other well characterized plant plasma membrane enzymes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1985-08-01 | Plant Science |