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RESEARCH PRODUCT

Viral Infections in Neonates with Suspected Late-Onset Bacterial Sepsis—A Prospective Cohort Study

Eva MildenbergerK. WeiseJulia WinterIsabella SchmehLena KleinAndré KidszunBritta GröndahlMarkus KnufMarkus KnufStephan Gehring

subject

MalevirusesBacteremiamedicine.disease_causeLate Onset DisordersAdenovirus Infections HumanCohort StudiesFeces0302 clinical medicineRotavirusGermanyNasopharynxBlood culture030212 general & internal medicineProspective StudiesCoronavirusCaliciviridae InfectionsParamyxoviridae InfectionsNeonatal sepsismedicine.diagnostic_testReverse Transcriptase Polymerase Chain ReactionObstetrics and Gynecologyvirus diseasesVirus DiseasesOriginal ArticleFemaleNeonatal SepsisCoronavirus InfectionsEnzyme-Linked Immunosorbent AssayvirusRespiratory Syncytial Virus InfectionsRotavirus InfectionsSepsis03 medical and health sciences030225 pediatricsIntensive Care Units NeonatalInfluenza HumanmedicineEnterovirus InfectionsHumanslate-onset bacterial sepsisPicornaviridae Infectionsbusiness.industryInfant Newbornmedicine.diseaseVirologyneonatal intensive care unitinfectionBlood CultureBacteremiaPediatrics Perinatology and Child HealthImmunologyNorovirusEnterovirusbusinessMultiplex Polymerase Chain Reaction

description

Objective The aim of our study was to evaluate the occurrence of viral infections in infants with suspected late-onset bacterial sepsis in a neonatal intensive care unit. Methods In a prospective study, infants with suspected late-onset bacterial sepsis underwent viral testing alongside routine blood culture sampling. Using a multiplex reverse transcription-polymerase chain reaction enzyme-linked immunosorbent assay, nasopharyngeal aspirates were analyzed for adenovirus, respiratory syncytial virus (RSV), influenza virus A and B, H1N1 virus, parainfluenza virus 1 to 4, metapneumovirus, coronavirus, and picornavirus. Stools were examined for adenovirus, rotavirus, norovirus, and enterovirus. Results Between August 2010 and March 2014, data of 88 infants with 137 episodes of suspected late-onset bacterial sepsis were analyzed. Six infants were diagnosed with a respiratory viral infection (2 × RSV, 4 × picornavirus). Blood culture–proven bacterial sepsis was detected in 15 infants. Neither viral–bacterial coinfections nor polymerase chain reaction positive stool samples were found. Conclusion Respiratory viruses can be detected in a considerable number of neonates with suspected late-onset bacterial sepsis. In contrast, gastrointestinal viral or enterovirus infections appear uncommon in such cases.

yearjournalcountryeditionlanguage
2016-05-01American Journal of Perinatology
10.1055/s-0036-1584150http://europepmc.org/articles/PMC7171717