6533b85bfe1ef96bd12bbf19
RESEARCH PRODUCT
Immunomorphological Pattern of Molecular Chaperones in Normal and Pathological Thyroid Tissues and Circulating Exosomes: Potential Use in Clinics
Antonella Marino GammazzaGiovanni TomaselloPier Luigi San BiagioAlberto J. L. MacarioAlessandro PitruzzellaCeleste Caruso BavisottoCeleste Caruso BavisottoGiuseppa GraceffaDaniela CabibiCalogero CipollaClaudia CampanellaBucchieri FabioRossana PorcasiRosario BaroneEverly Conway De MacarioDonatella BuloneFrancesca RappaFrancesco Cappellosubject
Male0301 basic medicineGoiterdiagnosismedicine.disease_causelcsh:Chemistry0302 clinical medicinelcsh:QH301-705.5Heat-Shock ProteinsSpectroscopygoiterbiologyThyroidmolecular chaperonesGeneral MedicineMiddle Agedmolecular chaperone3. Good healthComputer Science ApplicationsBlotdiagnosimedicine.anatomical_structure030220 oncology & carcinogenesisheat shock proteins (Hsp)ImmunohistochemistryFemalethyroid gland; papillary carcinoma; molecular chaperones; heat shock proteins (Hsp); goiter; exosomes; diagnosiendocrine systemanimal structuresexosomesArticleCatalysisInorganic Chemistry03 medical and health sciencesHsp27medicineHumansEndocrine systemexosomePhysical and Theoretical ChemistryMolecular Biologythyroid glandbusiness.industryOrganic Chemistrymedicine.diseaseCarcinoma PapillaryMicrovesicles030104 developmental biologylcsh:Biology (General)lcsh:QD1-999Cancer researchbiology.proteinCarcinogenesisbusinesspapillary carcinomadescription
The thyroid is a major component of the endocrine system and its pathology can cause serious diseases, e.g., papillary carcinoma (PC). However, the carcinogenic mechanisms are poorly understood and clinical useful biomarkers are scarce. Therefore, we determined if there are quantitative patterns of molecular chaperones in the tumor tissue and circulating exosomes that may be useful in diagnosis and provide clues on their participation in carcinogenesis. Hsp27, Hsp60, Hsp70, and Hsp90 were quantified by immunohistochemistry in PC, benign goiter (BG), and normal peritumoral tissue (PT). The same chaperones were assessed in plasma exosomes from PC and BG patients before and after ablative surgery, using Western blotting. Hsp27, Hsp60, and Hsp90 were increased in PC in comparison with PT and BG but no differences were found for Hsp70. Similarly, exosomal levels of Hsp27, Hsp60, and Hsp90 were higher in PC than in BG, and those in PC were higher before ablative surgery than after it. Hsp27, Hsp60, and Hsp90 show distinctive quantitative patterns in thyroid tissue and circulating exosomes in PC as compared with BG, suggesting some implication in the carcinogenesis of these chaperones and indicating their potential as biomarkers for clinical applications.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-09-01 | International Journal of Molecular Sciences |