6533b85cfe1ef96bd12bc041
RESEARCH PRODUCT
Expression and cellular localization of the Nef protein from human immunodeficiency virus-1 in stably transfected B-cells.
Norbert KienzleNikolaus Müller-lantzschMichael BachmannW. E. G. Müllersubject
Viral proteinvirusesGenetic VectorsFluorescent Antibody TechniqueBiologymedicine.disease_causeTransfectionVirusGene Products nefGene productAntigenVirologyGene expressionmedicineTumor Cells CulturedHumansnef Gene Products Human Immunodeficiency VirusCellular localizationB-LymphocytesMicroscopyvirus diseasesGeneral MedicineTransfectionVirologyMolecular biologyCytoplasmHIV-1Tetradecanoylphorbol Acetatedescription
Nef protein, encoded by the regulatory nef gene of human immunodeficiency virus type 1 (HIV-1), was expressed in the B-cell line Raji. The cells were stably transfected with plasmids containing the nef transcriptional cassette. They expressed Nef with an Mr of 27,000; the yield could be augmented by incubation with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. The intracellular localization of Nef was analyzed applying immunofluorescence microscopy using a confocal laser scanning microscope. The antigen was stained with a monoclonal antibody directed against the N-terminal part of Nef. The experiments revealed that in non-dividing cells Nef is present both in the cytoplasm and the nucleus while in dividing cells the viral protein is present in the cytoplasm and at the nuclear membrane.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-03-01 | Archives of virology |