6533b85cfe1ef96bd12bc1b0

RESEARCH PRODUCT

Entrapment of Citrus limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases

Octavio Díez-salesRita Abi RachedGiacomo Luigi PetrettoMansureh GhavamElvira Escribano-ferrerMaria ManconiJosé Esteban PerisSara FaisMaria Letizia MancaLucia PalmasFrancesca MarongiuMatteo AroffuGermano OrrùIris Usach

subject

0301 basic medicineLemonsSonicationkeratinocyte migrationlcsh:Medicinelcsh:RS1-441Pharmaceutical Sciencehydrogen peroxide02 engineering and technologyMalalties de la bocaCitral<i>Streptococcus mutans</i>law.inventionlcsh:Pharmacy and materia medica03 medical and health scienceschemistry.chemical_compoundlawPhosphatidylcholinescratch assayDrug DiscoveryLlimonesanticaries activityKeratinocyte migration<i>Citrus limon</i> essential oilHydrogen peroxideEssential oilLiposomeOlis essencialsChromatographyVesiclelcsh:RStreptococcusEstreptococs021001 nanoscience & nanotechnology030104 developmental biologyMouth diseaseschemistryEssences and essential oilsMolecular Medicine0210 nano-technology

description

This work aimed at developing a mouthwash based on liposomes loading Citrus limon var. pompia essential oil or citral to treat oropharyngeal diseases. Vesicles were prepared by dispersing phosphatidylcholine and pompia essential oil or citral at increasing amounts (12, 25 and 50 mg/mL) in water. Transparent vesicle dispersions were obtained by direct sonication avoiding the use of organic solvents. Cryogenic transmission electron microscopy (cryo-TEM) confirmed the formation of unilamellar, spherical and regularly shaped vesicles. Essential oil and citral loaded liposomes were small in size (~110 and ~100 nm, respectively) and negatively charged. Liposomes, especially those loading citral, were highly stable as their physico-chemical properties did not change during storage. The formulations were highly biocompatible against keratinocytes, were able to counteract the damages induced in cells by using hydrogen peroxide, and able to increase the rate of skin repair. In addition, liposomes loading citral at higher concentrations inhibited the proliferation of cariogenic bacterium.

yearjournalcountryeditionlanguage
2020-08-28
10.3390/ph13090216http://hdl.handle.net/2445/176075