6533b85cfe1ef96bd12bcbde

RESEARCH PRODUCT

The O-antigen of Plesiomonas shigelloides serotype O36 containing pseudaminic acid

Katarina StojkovicTomasz NiedzielaCzeslaw LugowskiMarta Kaszowska

subject

0301 basic medicineSerotypeMagnetic Resonance SpectroscopyStereochemistryMass spectrometrySerogroupBiochemistryAnalytical ChemistryLipid A03 medical and health sciencesResidue (chemistry)AntigenMALDI-TOF MSTrisaccharidechemistry.chemical_classification030102 biochemistry & molecular biologybiologyChemistryOrganic ChemistryO AntigensGeneral MedicineO-antigenbiology.organism_classificationPlesiomonas shigelloidesNMRMatrix-assisted laser desorption/ionization030104 developmental biologyBiochemistryCarbohydrate SequencePlesiomonas shigelloidesPlesiomonas

description

The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by 1H and 13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: →4)-β-Pse5Ac7(R3Hb)-(2 → 4)-β-D-Galp-(1 → 3)-β-D-GlcpNAc-(1→ These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the β-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs.2013, 11 (2), 440–454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434–10447).

yearjournalcountryeditionlanguage
2016-11-01Carbohydrate Research
10.1016/j.carres.2016.07.007https://doi.org/10.1016/j.carres.2016.07.007